Graduating in 1989 but having worked full time whilst studying for my degree part-time for 5 years, I started out my career as a Medical Laboratory Scientist, with specialisation in Clinical Biochemistry, in hospital and private pathology laboratories. Since then I have had some career breaks to raise a family of 4 but have always been very self-motivated and keen to work, and therefore have nearly always 'kept my hand in' with some kind of outside of the home work discipline. I also spent a significant period of time where I diversified from lab work, but remained in a technical role, as a Polysomnographic Technologist (sleep study technician). Since returning to my laboratory career in 2008 I have made the transition from pathology lab work to medical research lab work and have worked in different labs and roles during that time at Monash University (Clayton & Parkville). I have been a constant learner of new skills and techniques during these years and have thrived on it. I enjoy both the collegiate nature of research and the whole research environment, and also what it contributes to humanity. I am happy in my current position, but I am open to new challenges.More
- Providing research support for NH&MRC funded project grant into non-human primate brain structure and physiology.
Faculty of Medicine, Nursing and Health Sciences Monash University (Clayton campus) Overview of position description Provide research support to Rosa group in NeuroPhysiology, as directed by Dr. David Reser. Responsibilities/duties/skills: Prepare for and assist with brain surgeries, post-operative care, and euthanasia on non-human primate (NHP) animal models to place neuroanatomical tracer dyes, including handling of NHP subjects. Performing frozen microtome sectioning of fixed brain tissues, using a cryostat. Mounting of brain sections onto glass slides for further processing. Performing histological and immuno-histochemical stains on brain tissue sections (Gallyas stain for myelin, Nissl, BDA, Cytochrome oxidase). Preparation of chemical solutions and other items required for above processes. Microscopy and use of microscope linked software to draw brain sections, then identify and count neurons in sections containing fluorescent dyes and immuno-histochemical stains. Interfacing with computer software programs facilitating manipulation of digital images of stained sections to enable 3D reconstructions of brains - to permit analysis of results of surgeries. Management of `LabArchives' electronic notebook system, including conversion/uploading of paper-based records to digital format. Ordering processes and record keeping, including liaising with supplier representatives to obtain quotations. General lab maintenance and organisation.More
- Expression of desired proteins (bacterial & viral enzymes) from transformation of E. coli strains with plasmid construct vector, through large volume culture growth and induction of protein expression, to harvest of protein-containing bacterial cells. Proteins often labelled with 15N nitrogen or 13C carbon for further study with NMR.
- Purification of desired protein from harvested bacterial cells via AKTA Protein Chromatography.
- Identification of desired proteins using SDS-PAGE gels.
- Sterile technique. ;
- Making buffers and chemical solutions, antibiotic plates /aliquots, SDS-PAGE gels, and other items required for expression and purification process.
- Ordering processes and record keeping, including liaising with supplier representatives to obtain quotations.
- General lab maintenance and organisation.
Overview of position description: Assisting with supervised research specific work as directed by Assoc. Professor Martin Scanlon. Protein expression and purification using genetically modified bacterial strains to provide protein targets for Fragment Based Drug Design (FBDD) research projects. Maintenance of stocks of laboratory biological and chemical reagents and consumables for common use. Knowledge/Skills:More
- Reconciliation of Excel-based chemicals register with MSDS information in preparation for imminent chemicals audit.
- Physical organisation of chemicals storage to improve access.
- Paraffin embedding and microtome sectioning of animal tissues.
- Tissue culture techniques for stem cell propagation. ;
- Assisting with hormone assays. ;
- Assisting with preparation of supplies for animal surgeries. ;
- Observation of and assisting with sheep surgery. ;
- Record keeping duties.
Faculty of Medicine Nursing and Health Sciences Monash Institute of Medical Research Training ActivitiesMore
- Microtome sectioning of paraffin embedded animal tissues. ;
- Immunohistochemical staining of tissue sections. ;
- DNA isolation.
- Gel Electrophoresis.
Department of Anatomy & Developmental Biology School of Biomedical Sciences Monash University (Clayton) ResponsibilitiesMore
Sleep Disorders Centre (SDC) Mercy Private HospitalMore
Transferred when pathology services were outsourced to Biochemistry Department, Dorevitch Pathology.More
Education and Qualifications
|1989||Bachelors Degree - Applied Science||Other||Medical Laboratory||No|
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